Structure Prediction of Outer Membrane Protease Protein of Salmonella typhimurium Using Computational Techniques

Structure Prediction of Outer Membrane Protease Protein of Salmonella typhimurium Using Computational Techniques

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Salmonella typhimurium, a facultative gram-negative intracellular pathogen belonging to family Enterobacteriaceae, is the most frequent cause of human gastroenteritis worldwide.PgtE gene product, outer membrane protease emerges important in the intracellular phases of salmonellosis.The pgtE gene product of Table Cloth S.typhimurium was predicted to be capable of proteolyzing T7 RNA polymerase and localize in the outer membrane of these gram negative bacteria.

PgtE product of S.enterica and OmpT of E.coli, having high sequence similarity have been revealed to degrade macrophages, causing salmonellosis and other diseases.The three-dimensional structure of the protein was not available through Protein Data Bank (PDB) creating lack of structural information about E protein.

In our study, by performing Comparative model building, the three dimensional structure of outer membrane protease protein was generated using the backbone of the crystal structure of Pla of Yersinia pestis, retrieved from PDB, with MODELLER (9v8).Quality of the model was assessed by validation tool PROCHECK, web servers like ERRAT and DANDELION ROOT ProSA are used to certify the reliability of the predicted model.This information might offer clues for better understanding of E protein and consequently for developmet of better therapeutic treatment against pathogenic role of this protein in salmonellosis and other diseases.